Journal: BMC Biotechnology

Article Title: Oil body bound oleosin-rhFGF9 fusion protein expressed in safflower ( Carthamus tinctorius L.) stimulates hair growth and wound healing in mice

doi: 10.1186/s12896-018-0433-2

Figure Lengend Snippet: The effect of oil body bound oleosin-rhFGF9 of transgenic safflower was analyzing the activity of NIH/3 T3 cells. Various concentrations (0.82–212 ng/ml) of wild-type (WT) and oil body bound oleosin-rhFGF9 or rhFGF9 (FGF9 from E.coli) were used in NIH/3 T3 cells. DMEM was used as a blank control. Proliferation was quantified by measuring the absorbance at 570/630 nm

Article Snippet: A Human FGF9 ELISA Kit (Bioss) was used to measure oleosin-rhFGF9 concentration in the oil body protein, according to the manufacturer’s protocol.

Techniques: Transgenic Assay, Activity Assay

Journal: Biology of reproduction

Article Title: FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture.

doi: 10.1093/biolre/ioaa154

Figure Lengend Snippet: Figure 1. Identification of potential SRY binding sites in FGF9 promoter region. (A)

Article Snippet: FGF9 Enzyme-linked immunosorbent assay (ELISA) Culture medium was collected from SRY-overexpressing NT2/D1 cells and analyzed using the FGF9 ELISA kit (R&D Systems, USA).

Techniques: Binding Assay

Journal: Biology of reproduction

Article Title: FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture.

doi: 10.1093/biolre/ioaa154

Figure Lengend Snippet: Figure 2. Human SRY, not SF1, directly binds to FGF9 promoter and upregulates

Article Snippet: FGF9 Enzyme-linked immunosorbent assay (ELISA) Culture medium was collected from SRY-overexpressing NT2/D1 cells and analyzed using the FGF9 ELISA kit (R&D Systems, USA).

Techniques:

Journal: Biology of reproduction

Article Title: FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture.

doi: 10.1093/biolre/ioaa154

Figure Lengend Snippet: Figure 3. Mouse SRY and SF1 bind to Fgf9 promoter and cooperatively stimulate its

Article Snippet: FGF9 Enzyme-linked immunosorbent assay (ELISA) Culture medium was collected from SRY-overexpressing NT2/D1 cells and analyzed using the FGF9 ELISA kit (R&D Systems, USA).

Techniques:

Journal: Biology of reproduction

Article Title: FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture.

doi: 10.1093/biolre/ioaa154

Figure Lengend Snippet: Figure 4. FGF9 repressed ovarian genes and promoted testicular characteristics in

Article Snippet: FGF9 Enzyme-linked immunosorbent assay (ELISA) Culture medium was collected from SRY-overexpressing NT2/D1 cells and analyzed using the FGF9 ELISA kit (R&D Systems, USA).

Techniques:

Journal: Scientific Reports

Article Title: Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis

doi: 10.1038/s41598-019-56039-0

Figure Lengend Snippet: Metabolic stress induces FGF9 expression in NASH-fib. ( a ) Hepatic mRNA expression levels of Fgf9 in wild-type mice fed standard diet (normal liver), Col1a2-GFP Tg mice received intraperitoneal CCl 4 injection (CCl 4 liver), and MC4R-KO mice fed WD for 20 weeks (NASH liver) evaluated by quantitative real-time PCR. n = 6. ( b ) Protein expression levels of FGF9 in normal and NASH livers by Western blot analysis. Blots are shown as cropped images. Uncropped Western blot images are included in Supplementary Fig. . n = 3. * P < 0.05, ** P < 0.01 vs. normal liver; † P < 0.05 vs. CCl 4 liver. ( c ) Fgf9 mRNA expression levels in isolated HSCs and activated fibroblasts (CCl 4 -Fib, NASH-fib). n = 3. ( d ) Fgf9 mRNA expression levels in various cell types separated from normal and NASH livers. Resident macrophages, CD45 + Ly6G − F4/80 hi CD11b lo ; recruited macrophages, CD45 + Ly6G − F4/80 lo CD11b hi ; CD4 + T cells, CD45 + CD4 + ; and liver sinusoidal endothelial cells (LSEC), CD45 − CD146 + . Hepatocytes were isolated from lean wild-type mice and MC4R-KO mice fed WD for 4 weeks. n = 3–8. * P < 0.05 vs. HSCs; † P < 0.05 vs. CCl 4 -fib. ( e ) mRNA expression levels in cultured HSCs treated with TGFβ (10 ng/ml), lipopolysaccharide (LPS, 10 ng/ml), and palmitic acid (200 μM) for 24 hours. ( f ) Dose-dependent effect of palmitate (100, 200, and 500 μM) on FGF9 induction in HSCs. ( g ) Effect of various fatty acids (200 μM) on FGF9 induction. Lau, laurate; Ole, oleate. n = 5. * P < 0.05, ** P < 0.01 vs. veh; ## P < 0.01. Data represent mean ± SEM.

Article Snippet: The FGF9 concentration of the supernatants of these cells was measured by FGF9 Human ELISA Kit (RayBiotech, Norcross, GA, USA) according to the manufacture’s protocol.

Techniques: Expressing, Injection, Real-time Polymerase Chain Reaction, Western Blot, Isolation, Cell Culture

Journal: Scientific Reports

Article Title: Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis

doi: 10.1038/s41598-019-56039-0

Figure Lengend Snippet: FGF9 induces inflammatory changes in LX2 cells. Human HSC cell line LX2 cells were treated with human recombinant FGF9 at a dose of 1 or 10 ng/ml for 24 hours. ( a ) mRNA expression levels of proinflammatory cytokines ( IL1A , IL1B ), chemokines ( CCL2 , CXCL8 ) and fibrogenic factors ( COL1A1 , TGFB ). n = 4. * P < 0.05 vs. veh. FGF9 or GFP (control)-overexpressing LX2 cells (FGF9-LX2 and control-LX2, respectively) were established using lentiviral vectors. Western blot analysis ( b ) and FGF9 secretion ( c ) into culture supernatants using FGF9-overexpressing and control-LX2 cells. Blots are shown as cropped images. Uncropped Western blot images are included in Supplementary Fig. . n = 4. ** P < 0.01 vs. control-LX2. ( d ) mRNA expression levels of genes related to proinflammatory cytokines, chemokines and fibrogenic factors in FGF9-LX2 cells. n = 8. ** P < 0.01 vs. control-LX2. Data represent mean ± SEM.

Article Snippet: The FGF9 concentration of the supernatants of these cells was measured by FGF9 Human ELISA Kit (RayBiotech, Norcross, GA, USA) according to the manufacture’s protocol.

Techniques: Recombinant, Expressing, Western Blot

Journal: Scientific Reports

Article Title: Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis

doi: 10.1038/s41598-019-56039-0

Figure Lengend Snippet: FGF9 enhances cell migration and inhibits apoptosis in LX2 cells. ( a ) Effect of FGF9 on LX2 cell proliferation determined by WST assay after 96-hour incubation. n = 8. Effect of serum starvation (starve) for 48 hours ( b ) and coexistence with recombinant FGF9 (c) evaluated by caspase-3/7 activity assay in LX2 cells. n = 6. ( d ) Migration activity of FGF9-treated LX2 cells determined by transwell migration assay. LX2 cells were seeded onto the insert of transwell in serum free medium containing recombinant FGF9 (1 or 10 ng/ml), and incubated with medium containing 2% FBS in the lower chamber for 24 hours. n = 4. ** P < 0.01 vs. starve (−) or veh. n.s., not significant. Data represent mean ± SEM.

Article Snippet: The FGF9 concentration of the supernatants of these cells was measured by FGF9 Human ELISA Kit (RayBiotech, Norcross, GA, USA) according to the manufacture’s protocol.

Techniques: Migration, WST Assay, Incubation, Recombinant, Activity Assay, Transwell Migration Assay

Journal: Scientific Reports

Article Title: Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis

doi: 10.1038/s41598-019-56039-0

Figure Lengend Snippet: FGF9 enhances cell migration and inhibits apoptosis in HepG2 cells. ( a ) Effect of FGF9 on HepG2 cell proliferation determined by WST assay after 96-hour incubation. n = 8. Effect of anti-Fas antibody (Fas) at a dose of 100 ng/ml for 24 hours ( b ) and coexistence with recombinant FGF9 ( c ) evaluated by caspase-3/7 activity assay in HepG2 cells. n = 6. ( d ) Migration activity of FGF9-treated HepG2 cells determined by transwell migration assay. HepG2 cells were seeded onto the insert of transwell in serum free medium containing recombinant FGF9 (1 or 10 ng/ml), and incubated with medium containing 2% FBS in the lower chamber for 24 hours. n = 4. ** P < 0.01 vs. veh. n.s., not significant. Data represent mean ± SEM.

Article Snippet: The FGF9 concentration of the supernatants of these cells was measured by FGF9 Human ELISA Kit (RayBiotech, Norcross, GA, USA) according to the manufacture’s protocol.

Techniques: Migration, WST Assay, Incubation, Recombinant, Activity Assay, Transwell Migration Assay

Journal: Scientific Reports

Article Title: Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis

doi: 10.1038/s41598-019-56039-0

Figure Lengend Snippet: FGF9 promotes tumor growth in a human tumor xenograft model. HepG2 cells (2 × 10 5 cells) together with control-LX2 or FGF9-LX2 cells (1 × 10 6 cells) were transplanted subcutaneously in the flank of immunodeficient mice. ( a ) Time course of the tumor volume. Weight ( b ) and representative images ( c ) of subcutaneous tumors at 4 weeks after transplantation. αSMA staining ( d ) and TUNEL staining ( e ) of the tumors. Arrows indicate TUNEL-positive cells. ( f ) GFP and TUNEL double immunofluorescent staining of the tumors that includes HepG2 cells and control (GFP)-LX2 cells. ( g ) Ki67 immunostaining of the tumors. Scale bars: 100 μm. * P < 0.05, ** P < 0.01 vs. control. n = 7. Data represent mean ± SEM.

Article Snippet: The FGF9 concentration of the supernatants of these cells was measured by FGF9 Human ELISA Kit (RayBiotech, Norcross, GA, USA) according to the manufacture’s protocol.

Techniques: Transplantation Assay, Staining, TUNEL Assay, Immunostaining

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Mapping of the fibroblast growth factors in human white adipose tissue.

doi: 10.1210/jc.2009-2049

Figure Lengend Snippet: FIG. 3. Stimulation of in vitro differentiated adipocytes with FGFs. In vitro differentiated cells from sc WAT were stimulated for 10 min with different concentrations of FGF1, FGF2, FGF7, or FGF9 ranging from 0.1–10 ng/ml. Phosphorylated p44/42 (p44/42-P) was detected as a measure of FGF-induced cell activation. Untreated cells (c) were used as a negative control and phosphorylation-state-independent p44/42 was used as a control for sample variations.

Article Snippet: Medium was saved at 70 C for determination of FGF1, FGF2, FGF7, and FGF9 (DFA00B, DFB50, DKG00, and DY273, respectively; R&D Systems) levels by ELISA according to the manufacturer’s instructions.

Techniques: In Vitro, Activation Assay, Negative Control, Phospho-proteomics, Control